alpha Actinin 4 antibody (HRP)

Catalog No. ABIN733787

The Rabbit Polyclonal anti-alpha Actinin 4 antibody has been validated for WB and IHC (p). It is suitable to detect alpha Actinin 4 in samples from Human, Mouse and Rat.

Quick Overview for alpha Actinin 4 antibody (HRP) (ABIN733787)

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Actinin 4 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-alpha Actinin 4 Antibody (HRP)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Actinin alpha 4

Isotype: IgG

Application Details

Application Notes: WB: 1:100-1000, IHC-P: 1:100-500
Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for alpha Actinin 4

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Alternative Name: alpha Actinin 4

Background:

Synonyms: Actinin alpha 4, alpha-Actinin 4, alpha Actinin-4, actinin 4, actinin4, actinin-4, ACTN 4, ACTN4, DKFZp686K23158, F actin cross linking protein, FSGS 1, FSGS, FSGS1, Non muscle alpha actinin 4.

Background: Loading Control Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008].

Gene ID: 81

Pathways: Proton Transport