gamma H2AX antibody

Catalog No. ABIN7674615

The Rabbit Polyclonal anti-gamma H2AX antibody has been validated for WB, IHC and IF. It is suitable to detect gamma H2AX in samples from Human, Mouse and Rat.

Quick Overview for gamma H2AX antibody (ABIN7674615)

Target: gamma H2AX (GAMMA-H2AX) (gamma Histone Variant H2AX (GAMMA-H2AX))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This gamma H2AX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-gamma H2AX Antibody

Purpose: γ-H2AX antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: Peptide

Immunogen: A synthetic phosphorylated peptide around S139 of human H2AX

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:1000, IHC: 1:20-1:200, IF: 1:20-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for gamma H2AX

Target: gamma H2AX (GAMMA-H2AX) (gamma Histone Variant H2AX (GAMMA-H2AX))

Alternative Name: gamma-H2AX

Background:

Synonyms: Histone H2AX (H2a/x)|Histone H2A.X|H2AX|H2AFX

Background: H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability.

Molecular Weight: 17 kDa

Gene ID: 3014

UniProt: P16104