CD86 antibody (AA 140-175)

Catalog No. ABIN736701

This anti-CD86 antibody is a Rabbit Polyclonal antibody detecting CD86 in FACS, WB, ELISA, IHC (fro), ICC, IHC (p), IF (cc) and IF (p). Suitable for Human, Mouse, Rat and Dog. This Primary Antibody has been cited in 13+ publications.

Quick Overview for CD86 antibody (AA 140-175) (ABIN736701)

Target: CD86

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD86 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-CD86 Antibody

Binding Specificity: AA 140-175

Cross-Reactivity: Dog, Human, Mouse, Rat

Predicted Reactivity: Cow,Sheep,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat CD86

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CD86 antibody (ABIN736701)

Diehl, Kirchhoff, Hansmann: "CD86 is linked to apoptosis in canine histiocytic sarcoma." in: Frontiers in veterinary science, Vol. 12, pp. 1546047, (2025) (PubMed).

Lee, Yu, Trimpert, Benthani, Mairhofer, Richter-Pechanska, Wyler, Belenki, Kaltenbrunner, Pammer, Kausche, Firsching, Dietert, Schotsaert, Martínez-Romero, Singh, Kunz, Niemeyer, Ghanem, Salzer, Paar et al.: "Virus-induced senescence is a driver and therapeutic target in COVID-19. ..." in: Nature, Vol. 599, Issue 7884, pp. 283-289, (2021) (PubMed).

Han, Wang, Qin, Xia, Wu: "LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4." in: Journal of experimental & clinical cancer research : CR, Vol. 36, Issue 1, pp. 83, (2018) (PubMed).

Chen, Zhang, Wang, Chen, Yan, Zhang, Zhang: "Peptide-modified chitosan hydrogels promote skin wound healing by enhancing wound angiogenesis and inhibiting inflammation." in: American journal of translational research, Vol. 9, Issue 5, pp. 2352-2362, (2017) (PubMed).

Zhang, Zhang, Yin, Li, Bao, Chen: "Variation and significance of secretory immunoglobulin A, interleukin 6 and dendritic cells in oral cancer." in: Oncology letters, Vol. 13, Issue 4, pp. 2297-2303, (2017) (PubMed).

Gerzanich, Makar, Guda, Kwon, Stokum, Woo, Ivanova, Ivanov, Mehta, Morris, Bryan, Bever, Simard: "Salutary effects of glibenclamide during the chronic phase of murine experimental autoimmune encephalomyelitis." in: Journal of neuroinflammation, Vol. 14, Issue 1, pp. 177, (2017) (PubMed).

Xie, Wu, Zhou, Yang, Tian, Huang, Meng, Li: "PICK1 confers anti-inflammatory effects in acute liver injury via suppressing M1 macrophage polarization." in: Biochimie, Vol. 127, pp. 121-32, (2016) (PubMed).

Das, Singh, Majid, Sherman, Mukhopadhyay, Wright, Martin, Dunn, Baker: "Syndesome Therapeutics for Enhancing Diabetic Wound Healing." in: Advanced healthcare materials, Vol. 5, Issue 17, pp. 2248-60, (2016) (PubMed).

Chen, Li, Zhao, Liang, Ma, Li, Zhu: "Prevention of ultraviolet radiation‑induced immunosuppression by sunscreen in Candida albicans‑induced delayed‑type hypersensitivity." in: Molecular medicine reports, Vol. 14, Issue 1, pp. 202-8, (2016) (PubMed).

Ju, Xing, Yang, Zheng, Jia, Zhang, Ren: "An effective cytokine adjuvant vaccine induces autologous T-cell response against colon cancer in an animal model." in: BMC immunology, Vol. 17, Issue 1, pp. 31, (2016) (PubMed).

Chen, Shi, Zhang, Chen, Wang, Zhang, Tian, Yan, Li, Zhong, Xing, Zhang, Zhang: "Mesenchymal stem cell-laden anti-inflammatory hydrogel enhances diabetic wound healing." in: Scientific reports, Vol. 5, pp. 18104, (2016) (PubMed).

Evans, Jonason, Bussler, Torno, Veeraraghavan, Reilly, Doherty, Seils, Winter, Mallow, Kirk, Howell, Giralico, Scrivens, Klimatcheva, Fisher, Bowers, Paris, Smith, Zauderer: "Antibody blockade of semaphorin 4D promotes immune infiltration into tumor and enhances response to other immunomodulatory therapies." in: Cancer immunology research, (2015) (PubMed).

Gao, Zhang, Ding, Chen, Hu, Jiang, Li, Chen, Wang, Ye, Zhu: "Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells." in: Cell transplantation, Vol. 22, Issue 10, pp. 1883-900, (2014) (PubMed).

Target Details for CD86

Target: CD86

Alternative Name: CD86

Background:

Synonyms: B7-2, Cd86 antigen, Cd86, Membrane glycoprotein

Background: This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.

Gene ID: 56822

UniProt: O35531

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation