Recombinant CD3 antibody

Catalog No. ABIN7795536

The Mouse Bispecific anti-CD3 antibody has been validated for Func, ELISA, Neut and IGA. It is suitable to detect CD3 in samples from Human.

Quick Overview for Recombinant CD3 antibody (ABIN7795536)

Target: CD3

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Bispecific

Conjugate: This CD3 antibody is un-conjugated

Application: Functional Studies (Func), ELISA, Neutralization (Neut), Immunogenicity Assay (IGA)

Grade: In vivo Grade

Clone: SP34-2-OKT3

Product Details anti-CD3 Antibody

Expression System: Mammalian cells

Purpose: In Vivo Grade Recombinant Anti-human CD3 / CD3 Bispecific Antibody, Mouse IgG1 lambda (Clone SP34-2 / OKT3)

Characteristics: The OKT3 antibody binds to CD3 epsilon of the T cell receptor-CD3 complex found only on mature T cells and medullary thymocytes. An interaction between T cells, OKT3, and monocytes causes T-cell activation (mitogenesis) in vitro. On the other hand, the OKT3 antibody blocks the unique association between the antigen receptor and CD3, and both the generation and function of cytotoxic T cells. After an initial dose of the OKT3 antibody, T cells disappear from the circulation within minutes to hours. During treatment with the OKT3 antibody, T cells bearing the usual array of surface molecules (CD2, CD4, and CD8) reappear in the peripheral blood circulation but these cells are devoid of CD3. After another 48 hours without the OKT3 antibody, the normal array of surface molecules including CD3 appear again on all T cells. The selective removal of CD3 by internalization is thought to be the key mechanism of action of the OKT3 antibody. Comodulation of the antigen receptor with CD3 explains the immunoblocking action of the OKT3 antibody in vivo. The OKT3 antibody is ineffective clinically if the CD3 target molecule is not modulated. The OKT3 antibody is effective for induction of immunosuppression and for treating initial and steroid-resistant rejections. The anti-human CD3 antibody (clone: SP34-2), a derivative of SP34, is known to cross-react with a major subset of peripheral blood lymphocytes in both human and non-human primates, including baboon, rhesus, cynomolgus, and pigtail macaque monkeys. The SP34-2 antibody specifically recognizes a major subset of CD3-positive cells from non-human primates, similar to its human counterpart, but does not react with monocytes or granulocytes. The SP34-2 antibody is used in flow cytometry to identify and analyze CD3-positive cells in human and various non-human primate species. It can also be used to stimulate cell proliferation in various primate peripheral blood mononuclear cells (PBMC). Our recombinant anti-human OKT3 / SP34-2 bispecific antibodies have a part (variable regions) or complete amino acid sequences of the mouse anti-human CD3 antibody (hybridoma clone name or number: OKT3 and SP34-2). It is under investigation whether either of the recombinant anti-human CD3 / CD3 bispecific antibodies (OKT3 / SP34-2 and SP34-2 / OKT3) is the best anti-human CD3 antibody.

Purification: Protein A or G affinity column

Purity: >95 % by reducing SDS-PAGE

Endotoxin Level: < 1 EU per 1 mg of the protein by the LAL method

Isotype: IgG1, lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: > 3 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: Short time 2 to 8°C as supplied. Long time -20°C to -70°C as supplied.

Target Details for CD3

Target: CD3

Alternative Name: CD3 & CD3

Sub Type: Bispecific

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway