Recombinant EIF4E antibody (pSer209)

Catalog No. ABIN7798529

This Rabbit Monoclonal antibody specifically detects EIF4E in WB and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant EIF4E antibody (pSer209) (ABIN7798529)

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EIF4E antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Grade: KD Validated

Clone: 25GB3235

Product Details anti-EIF4E Antibody

Binding Specificity: pSer209

Expression System: HEK-293 Cells

Purpose: KD-Validated Phospho-eIF4E (S209) Recombinant Rabbit mAb

Cross-Reactivity: Mouse, Rat

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human Phospho-eIF4E (S209)

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:2,000-1:10,000, Flow Cytometry (FCM): 1:200-1:2,000

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for EIF4E

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Alternative Name: eIF4E

Molecular Weight: 25 kDa

Gene ID: 1977

UniProt: P06730

Pathways: BCR Signaling