ADRP antibody (AA 31-130)

Catalog No. ABIN738606

This anti-ADRP antibody is a Rabbit Polyclonal antibody detecting ADRP in WB, ELISA, IHC (p), IF (p), IF (cc) and IHC (fro). Suitable for Human and Mouse. This Primary Antibody has been cited in 4+ publications.

Quick Overview for ADRP antibody (AA 31-130) (ABIN738606)

Target: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADRP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ADRP Antibody

Binding Specificity: AA 31-130

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat ADFP

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-ADRP antibody (ABIN738606)

Zhao, Han, Hong, Sun: "Adipose differentiation‑related protein knockdown inhibits vascular smooth muscle cell proliferation and migration and attenuates neointima formation." in: Molecular medicine reports, Vol. 16, Issue 3, pp. 3079-3086, (2018) (PubMed).

Amrutkar, Cansby, Nuñez-Durán, Pirazzi, Ståhlman, Stenfeldt, Smith, Borén, Mahlapuu: "Protein kinase STK25 regulates hepatic lipid partitioning and progression of liver steatosis and NASH." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 29, Issue 4, pp. 1564-76, (2015) (PubMed).

Amrutkar, Cansby, Chursa, Nuñez-Durán, Chanclón, Ståhlman, Fridén, Mannerås-Holm, Wickman, Smith, Bäckhed, Borén, Howell, Mahlapuu: "Genetic Disruption of Protein Kinase STK25 Ameliorates Metabolic Defects in a Diet-Induced Type 2 Diabetes Model." in: Diabetes, Vol. 64, Issue 8, pp. 2791-804, (2015) (PubMed).

Liu, Dai, Liu, Liu, Tang, Wang, Yi, Liu, Jiang, Yang, Yuan: "Oxidized low-density lipoprotein activates adipophilin through ERK1/2 signal pathway in RAW264.7 cells." in: Acta biochimica et biophysica Sinica, Vol. 42, Issue 9, pp. 635-45, (2010) (PubMed).

Target Details for ADRP

Target: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Alternative Name: ADFP

Background:

Synonyms: ADFP, Adipophilin, Adipose dferentiation related protein, Adipose dferentiation-related protein, Perilipin-2, PLIN2, PLIN2_HUMAN, adipophilin, Adipose dferentiation related protein, ADRP, MGC10598

Background: Milk lipid globules from humans, cows and rats contain a protein identified as adipocyte differentiation-related protein (ADFP) associated with the globule surface membrane material. This protein, previously believed to be specific to adipocytes, was a major constituent of the globule surface and was present in a detergent-insoluble complex that contained stoichiometric amounts of butyrophilin and xanthine oxidase. ADFP (Adipophilin) occurs in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells. In tissues, however, expression of adipophilin is restricted to certain cell types, such as lactating mammary epithelial cells, adrenal cortex cells, Sertoli and Leydig cells of the male reproductive system, and steatosis or fatty change hepatocytes in alcoholic liver cirrhosis. ADFP may be a possible new marker for the identification of specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells.

Gene ID: 298199

Pathways: Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism