Atp5f1b (AA 112-517) antibody

Catalog No. ABIN7825276

The Rabbit Polyclonal anti-Atp5f1b antibody has been validated for WB, IHC and IF. It is suitable to detect Atp5f1b in samples from Zebrafish (Danio rerio).

Quick Overview for Atp5f1b (AA 112-517) antibody (ABIN7825276)

Target: Atp5f1b

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details

Binding Specificity: AA 112-517

Purpose: Anti-Zebrafish ATPB/ATP5F1B Antibody

Characteristics: Anti-Zebrafish ATPB/ATP5F1B Antibody catalog # AZA8WGC6. Tested in WB, IHC, IF applications. This antibody reacts with Zebrafish. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived zebrafish ATPB/ATP5F1B recombinant protein (Position: Q112-S517).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Zebrafish
Immunohistochemistry, 2-5 μg/mL, Zebrafish
Immunofluorescence, 5 μg/mL, Zebrafish

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details

Target: Atp5f1b

Background: This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.