NANOS3 antibody (AA 1-159)

Catalog No. ABIN7825582

The Rabbit Polyclonal anti-NANOS3 antibody has been validated for IHC. It is suitable to detect NANOS3 in samples from Zebrafish (Danio rerio).

Quick Overview for NANOS3 antibody (AA 1-159) (ABIN7825582)

Target: NANOS3 (Nanos Homolog 3 (NANOS3))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NANOS3 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-NANOS3 Antibody

Binding Specificity: AA 1-159

Purpose: Anti-Zebrafish NANOS3 Antibody

Characteristics: Anti-Zebrafish NANOS3 Antibody catalog #AZQ90WW1. Tested in IHC applications. This antibody reacts with Zebrafish.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived zebrafish NANOS3 recombinant protein (Position: M1-W159)

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry, 2-5 μg/mL, Zebrafish

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NANOS3

Target: NANOS3 (Nanos Homolog 3 (NANOS3))

Alternative Name: NANOS3

Background: Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In Drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2-null primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3-null PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3-null background. Nanos3 expression can not rescue Nanos2-null defects.

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling