PI4KB antibody (AA 394-690)

Catalog No. ABIN7825680

This Rabbit Polyclonal antibody specifically detects PI4KB in IHC. It exhibits reactivity toward Zebrafish (Danio rerio).

Quick Overview for PI4KB antibody (AA 394-690) (ABIN7825680)

Target: PI4KB (Phosphatidylinositol 4-Kinase, Catalytic, beta (PI4KB))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PI4KB antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-PI4KB Antibody

Binding Specificity: AA 394-690

Purpose: Anti-Zebrafish PI4KB Antibody

Characteristics: Anti-Zebrafish PI4KB Antibody catalog # AZQ49GP3. Tested in IHC applications. This antibody reacts with Zebrafish.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived zebrafish PI4KB recombinant protein (Position: D394-D690).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry, 2-5 μg/mL, Zebrafish

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PI4KB

Target: PI4KB (Phosphatidylinositol 4-Kinase, Catalytic, beta (PI4KB))

Alternative Name: PI4KB

Background: Phosphatidylinositol 4-kinase beta is an enzyme that in humans is encoded by the PI4KB gene. Inositol phospholipids have an important role in intracellular signaling in response to hormones, growth factors and neurotransmitters. Phosphatidylinositol 4-kinase phosphorylates phosphatidylinositol (PI) to phosphatidylinositol-4-phosphate (PIP). In a second step, PIP is further phosphorylated to phosphatidylinositol-4,5-bisphosphate (PIP2), and PIP2 is subsequently hydrolyzed by phospholipase C, producing the two intracellular second messengers, inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). PI4K230, PI4K92 and PI4K55 are three PI4 kinase isoforms that have been characterized and classified according to their molecular weights of 230, 92 and 55 kD. Previously, PI4 kinases were classified into type II and III enzymes. All isoforms are located on distinct membranes and cellular compartments suggesting various tasks. PI4K230 is located at the endoplasmatic reticulum and outer membranes of mitochondria, PI4K92 at the Golgi apparatus and endoplasmatic reticulum, and PI4K55 at the plasma membrane and endosomes. PI4K230 is predominantly expressed in brain and moderately sensitive to wortmannin as well as specifically and irreversibly inhibited by cyclitol derivatives.

Pathways: Inositol Metabolic Process