Dnmt2 antibody (AA 16-391)

Catalog No. ABIN7825990

The Rabbit Polyclonal anti-Dnmt2 antibody has been validated for ELISA, WB and FACS. It is suitable to detect Dnmt2 in samples from Mouse and Rat.

Quick Overview for Dnmt2 antibody (AA 16-391) (ABIN7825990)

Target: Dnmt2 (TRDMT1) (tRNA Aspartic Acid Methyltransferase 1 (TRDMT1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dnmt2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-Dnmt2 Antibody

Binding Specificity: AA 16-391

Purpose: Anti-DNMT2/TRDMT1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-DNMT2/TRDMT1 Antibody catalog # A06504-1. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse DNMT2/TRDMT1 recombinant protein (Position: M16-E391). Mouse DNMT2/TRDMT1 shares 80.6% and 88% amino acid (aa) sequence identity with human and rat DNMT2/TRDMT1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Dnmt2

Target: Dnmt2 (TRDMT1) (tRNA Aspartic Acid Methyltransferase 1 (TRDMT1))

Alternative Name: TRDMT1

Background: Enables tRNA (cytidine-5-)-methyltransferase activity. Involved in tRNA methylation and tRNA stabilization. Predicted to be located in cytoplasm. Predicted to be active in nucleus. Is expressed in several structures, including central nervous system, genitourinary system, hemolymphoid system gland, olfactory epithelium, and thyroid gland. Orthologous to human TRDMT1 (tRNA aspartic acid methyltransferase 1).

Molecular Weight: 55 kDa

Gene ID: 13434

UniProt: O55055