CD163 antibody (AA 1001-1121) (FITC)

Catalog No. ABIN741577

This Rabbit Polyclonal antibody specifically detects CD163 in WB, FACS, IF (cc) and IF (p). It exhibits reactivity toward Human, Mouse, Rat, Pig and Dog and has been mentioned in 3+ publications.

Quick Overview for CD163 antibody (AA 1001-1121) (FITC) (ABIN741577)

Target: CD163

Reactivity: Human, Mouse, Rat, Pig, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD163 antibody is conjugated to FITC

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-CD163 Antibody (FITC)

Binding Specificity: AA 1001-1121

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Predicted Reactivity: Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CD163/M130

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-CD163 antibody (FITC) (ABIN741577)

Kobori, Hamasaki, Kitaura, Yamazaki, Nishinaka, Niwa, Nakao, Wake, Mori, Yoshino, Nishibori, Takahashi: "Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis." in: Frontiers in immunology, Vol. 9, pp. 334, (2019) (PubMed).

Oh, Riek, Zhang, Williams, Darwech, Bernal-Mizrachi: "Deletion of JNK2 prevents vitamin-D-deficiency-induced hypertension and atherosclerosis in mice." in: The Journal of steroid biochemistry and molecular biology, Vol. 177, pp. 179-186, (2017) (PubMed).

Weng, Sprague, Oh, Riek, Chin, Garcia, Bernal-Mizrachi: "Vitamin D deficiency induces high blood pressure and accelerates atherosclerosis in mice." in: PLoS ONE, Vol. 8, Issue 1, pp. e54625, (2013) (PubMed).

Target Details for CD163

Target: CD163

Alternative Name: Cd163/M130

Background:

Synonyms: M13, MM13, Scavenger receptor cysteine-rich type 1 protein M13, Hemoglobin scavenger receptor, CD163, M130

Background: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH -dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

Gene ID: 9332

UniProt: Q86VB7