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Histone 3 antibody (pSer28)

Name: Histone 3 antibody (pSer28)
SKU: ABIN742568
Price: 384.62 USD
Availability: InStock

The Rabbit Polyclonal anti-Histone 3 antibody has been validated for WB, ELISA, IHC (p) and FACS. It is suitable to detect Histone 3 in samples from Human and Rat.

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Phospho-Histone H3 (Ser28) Polyclonal Antibody, Unconjugated (ABIN742568) at 1:200 followed by conjugation to the secondary antibody and DAB staining (Histone 3 antibody (pSer28))

IHC

Hela cells were fixed with 70% ice-cold methanol overnight at 4℃, permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. (Histone 3 antibody (pSer28))

2 images

Catalog No. ABIN742568

Quick Overview for Histone 3 antibody (pSer28) (ABIN742568)

Target

See all Histone 3 (H3) Antibodies

Histone 3 (H3) (Histone H3 (H3))

Reactivity

1429
938
838
39
39
37
34
32
25
23
20
19
11
6
5
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1

Human, Rat

Host

Rabbit

Clonality

Polyclonal

Conjugate

This Histone 3 antibody is un-conjugated

Application

1078
390
387
340
248
191
188
173
163
157
146
129
112
48
48
37
35
26
25
15
14
7
7
7
4
3
1
1
1
1

Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Discover our top product H3 Primary Antibody

Binding Specificity
51

50

49

45

42

40

38

37

36

36

36

34

32

32

32

30

29

26

24

23

22

19

19

18

18

18

17

17

15

15

15

15

15

14

14

13

12

12

12

12

12

11

11

10

10

9

9

9

8

8
pSer28

Specificity

This phosphorylation site is homologous across the listed species.

Cross-Reactivity

Human, Rat

Predicted Reactivity

Mouse,Cow,Pig,Rabbit,Drosophila

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser29

Isotype

IgG
anti-Histone H3 (H3) (H3K4me) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (3meLys4) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 Reactivity: Human, Mouse WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 Reactivity: Human, Mouse WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Mouse Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, IHC (p), IF (cc), FACS, IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me3) antibody
H3 Reactivity: Human, Mouse, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (C-Term) antibody
H3 Reactivity: Human WB, IHC, ChIP, IP Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9me2) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me2) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

Histone 3 (H3) (Histone H3 (H3))

Alternative Name

Histone H3

Background

Synonyms: H3t, H3.4, H3/g, H3FT, Histone H3.1t, H3/t, HIST3H3
Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene ID

8290

UniProt

Q16695