LRP6 antibody (pSer1490)

Catalog No. ABIN743558

The Rabbit Polyclonal anti-LRP6 antibody has been validated for WB, ELISA, IF (p), IF (cc), IHC (p) and IHC (fro). It is suitable to detect LRP6 in samples from Human, Mouse and Rat. There are 3+ publications available.

Quick Overview for LRP6 antibody (pSer1490) (ABIN743558)

Target: LRP6 (Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LRP6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-LRP6 Antibody

Binding Specificity: pSer1490

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human LRP6 around the phosphorylation site of Ser1490

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-LRP6 antibody (ABIN743558)

Xiao, Fei, Hurley: "FGF2 crosstalk with Wnt signaling in mediating the anabolic action of PTH on bone formation." in: Bone reports, Vol. 9, pp. 136-144, (2018) (PubMed).

Boone, Arend, Johnston, Cooper, Gilchrist, Oelschlager, Grizzle, McGwin, Gangrade, Straughn, Buchsbaum: "Targeting the Wnt/β-catenin pathway in primary ovarian cancer with the porcupine inhibitor WNT974." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 96, Issue 2, pp. 249-59, (2016) (PubMed).

Chen, Xu, Ye, Li, Gong, Zhang, Wu, Jia, Liu, Chen, Yang, Tang, Zhu, Ge, Zou: "Urotensin II inhibited the proliferation of cardiac side population cells in mice during pressure overload by JNK-LRP6 signalling." in: Journal of cellular and molecular medicine, Vol. 18, Issue 5, pp. 852-62, (2014) (PubMed).

Target Details for LRP6

Target: LRP6 (Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))

Alternative Name: LRP6

Background:

Synonyms: ADCAD2, Low-density lipoprotein receptor-related protein 6, LRP-6, LRP6

Background: Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation (By similarity).

Gene ID: 4040

UniProt: O75581

Pathways: WNT Signaling, Tube Formation