Troponin I antibody (pSer23, pSer24) (HRP)

Catalog No. ABIN746642

The Rabbit Polyclonal anti-Troponin I antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect Troponin I in samples from Rat.

Quick Overview for Troponin I antibody (pSer23, pSer24) (HRP) (ABIN746642)

Target: Troponin I (TNNI)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Troponin I antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Troponin I Antibody (HRP)

Binding Specificity: pSer23, pSer24

Specificity: These phosphorylation sites are homologous among the listed cross-reactive species.

Cross-Reactivity: Rat

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Troponin I around the phosphorylation site of Ser23/24

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Troponin I

Target: Troponin I (TNNI)

Alternative Name: Troponin I +

Background:

Synonyms: ANM, TNT, NEM5, STNT, TNTS, Troponin T, slow skeletal muscle, Slow skeletal muscle troponin T, TNNT1

Background: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Gene ID: 7138

UniProt: P13805