NUMA1 antibody (AA 1001-1200) (Cy5.5)

Catalog No. ABIN747433

This Rabbit Polyclonal antibody specifically detects NUMA1 in IF (p) and IF (cc). It exhibits reactivity toward Human.

Quick Overview for NUMA1 antibody (AA 1001-1200) (Cy5.5) (ABIN747433)

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUMA1 antibody is conjugated to Cy5.5

Application: Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-NUMA1 Antibody (Cy5.5)

Binding Specificity: AA 1001-1200

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NuMA

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NUMA1

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Alternative Name: NuMA

Background:

Synonyms: NUMA 1, NuMA protein, NUMA1, SP H antigen, Structural nuclear protein, Centrophilin stabilizes mitotic spindle in mitotic cells, Nuclear mitotic apparatus protein 1, Nuclear mitotic apparatus protein, NUMA1_HUMAN.

Background: NuMA (Nuclear Mitotic Apparatus Protein) is an intranuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200 kDa during apoptosis.

Gene ID: 4926

UniProt: Q14980

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, M Phase