PGE2 antibody (AA 281-384)

Catalog No. ABIN748403

The Rabbit Polyclonal anti-PGE2 antibody has been validated for FACS, IF (cc), IF (p), ELISA, IHC (fro) and IHC (p). It is suitable to detect PGE2 in samples from Human, Mouse and Rat. There are 4+ publications available.

Quick Overview for PGE2 antibody (AA 281-384) (ABIN748403)

Target: PGE2 (Prostaglandin E2 (PGE2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PGE2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PGE2 Antibody

Binding Specificity: AA 281-384

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse PGES2

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-PGE2 antibody (ABIN748403)

Manaserh, Chikkamenahalli, Ravi, Dube, Park, Hill: "Ablating astrocyte insulin receptors leads to delayed puberty and hypogonadism in mice." in: PLoS biology, Vol. 17, Issue 3, pp. e3000189, (2019) (PubMed).

Chia, Liu, Feng, Tseng, Hung, Chen, Tan: "The Antinociceptive Effect of Light-Emitting Diode Irradiation on Incised Wounds Is Correlated with Changes in Cyclooxygenase 2 Activity, Prostaglandin E2, and Proinflammatory Cytokines." in: Pain research & management, Vol. 2017, pp. 4792489, (2017) (PubMed).

Liu, Li, Liu, She, Shi, Li, Wang, Jiang: "Activated hepatic stellate cells directly induce pathogenic Th17 cells in chronic hepatitis B virus infection." in: Experimental cell research, Vol. 359, Issue 1, pp. 129-137, (2017) (PubMed).

de Jesus, Spadacci-Morena, dos Anjos Rabelo, Pinfildi, Fukuda, Plapler: "Low-level laser therapy in IL-1β, COX-2, and PGE2 modulation in partially injured Achilles tendon." in: Lasers in medical science, Vol. 30, Issue 1, pp. 153-8, (2015) (PubMed).

Target Details for PGE2

Target: PGE2 (Prostaglandin E2 (PGE2))

Background:

Synonyms: Gbf1, Pges2, C79137, Mpges2, 6138H1Rik, Prostaglandin E synthase 2, GATE-binding factor 1, GBF-1, Microsomal prostaglandin E synthase 2, mPGES-2, Ptges2,

Background: Isomerase that catalyzes the conversion of PGH2 into the more stable prostaglandin E2 (PGE2) (By similarity). May also have transactivation activity toward IFN-gamma (IFNG), possibly via an interaction with CEBPB, however, the relevance of transcription activation activity remains unclear.

Gene ID: 96979

UniProt: Q8BWM0