MT-ND5 antibody (AA 451-550)

Catalog No. ABIN751633

This Rabbit Polyclonal antibody specifically detects MT-ND5 in WB, ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). It exhibits reactivity toward Human and Ratand has been mentioned in 1 publication.

Quick Overview for MT-ND5 antibody (AA 451-550) (ABIN751633)

Target: MT-ND5 (Mitochondrially Encoded NADH Dehydrogenase 5 (MT-ND5))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MT-ND5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MT-ND5 Antibody

Binding Specificity: AA 451-550

Cross-Reactivity: Human, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MT-ND5

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-MT-ND5 antibody (ABIN751633)

Wu, Zhang, Nickerson, Gao, Sun, Chen, Zhang, Zhang, Gao, Chen, Luo, Wang, Sun: "Cumulative mtDNA damage and mutations contribute to the progressive loss of RGCs in a rat model of glaucoma." in: Neurobiology of disease, Vol. 74, pp. 167-79, (2015) (PubMed).

Target Details for MT-ND5

Target: MT-ND5 (Mitochondrially Encoded NADH Dehydrogenase 5 (MT-ND5))

Alternative Name: MT-ND5

Background:

Synonyms: MTND5, NADH-ubiquinone oxidoreductase chain 5, NADH dehydrogenase subunit 5, MT-ND5, NADH5, ND5

Background: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).

Gene ID: 4540

UniProt: P03915