FE65 antibody (pSer175)

Catalog No. ABIN755797

This Rabbit Polyclonal antibody specifically detects FE65 in WB, IF (cc), IF (p), ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Mouse.

Quick Overview for FE65 antibody (pSer175) (ABIN755797)

Target: FE65 (APBB1) (Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 1 (Fe65) (APBB1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FE65 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-FE65 Antibody

Binding Specificity: pSer175

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human APBB1 around the phosphorylation site of Ser175 [LS(p-S)PP]

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for FE65

Target: FE65 (APBB1) (Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 1 (Fe65) (APBB1))

Alternative Name: APBB1

Background:

Synonyms: Adaptor protein FE65a2, Amyloid beta A4 precursor protein binding family B member 1, Amyloid beta A4 precursor protein binding family B, Amyloid beta A4 precursor protein binding family B member 1, Amyloid beta precursor protein binding family B member 1, APBB 1, APBB1, FE 65, Fe65 protein, MGC 9072, MGC9072, Protein Fe65, RIR, Stat like protein, Fe-65 Protein, APBB1_HUMAN.

Background: The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Gene ID: 322

Pathways: Positive Regulation of Response to DNA Damage Stimulus