PHAP antibody (AA 41-140)

Catalog No. ABIN762176

The Rabbit Polyclonal anti-PHAP antibody has been validated for WB, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) and IP. It is suitable to detect PHAP in samples from Human, Mouse and Rat. There is 1 publication available.

Quick Overview for PHAP antibody (AA 41-140) (ABIN762176)

Target: PHAP (ANP32A/ANP32B) (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member A/B (ANP32A/ANP32B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHAP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Product Details anti-PHAP Antibody

Binding Specificity: AA 41-140

Specificity: Will recognize Acidic leucine-rich nuclear phosphoprotein 32 family member C,D, and E

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PHAP1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
IP(1-2 μg)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-PHAP antibody (ABIN762176)

Hung, Flint: "Normal human cell proteins that interact with the adenovirus type 5 E1B 55kDa protein." in: Virology, Vol. 504, pp. 12-24, (2017) (PubMed).

Target Details for PHAP

Target: PHAP (ANP32A/ANP32B) (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member A/B (ANP32A/ANP32B))

Alternative Name: PHAP

Background:

Synonyms: Acidic leucine-rich nuclear phosphoprotein 32 family member A, Acidic nuclear phosphoprotein pp32, pp32, Leucine-rich acidic nuclear protein, LANP, Mapmodulin, Potent heat-stable protein phosphatase 2A inhibitor I1PP2A, Putative HLA-DR-associated protein I, PHAPI, ANP32A, C15orf1, MAPM, PHAP1

Background: Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression.

Gene ID: 8125

UniProt: P39687