SPARCL1 antibody (AA 601-664)

Catalog No. ABIN762581

The Rabbit Polyclonal anti-SPARCL1 antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect SPARCL1 in samples from Rat and Mouse. There is 1 publication available.

Quick Overview for SPARCL1 antibody (AA 601-664) (ABIN762581)

Target: SPARCL1 (SPARC-Like 1 (Hevin) (SPARCL1))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPARCL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SPARCL1 Antibody

Binding Specificity: AA 601-664

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SPARCL1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-SPARCL1 antibody (ABIN762581)

Lai, Wu, Zou, Xie, Zhang, Zhao, Zhao, Wang, Ji: "Secretome Analyses of Aβ(1-42) Stimulated Hippocampal Astrocytes Reveal that CXCL10 is Involved in Astrocyte Migration." in: Journal of proteome research, (2013) (PubMed).

Target Details for SPARCL1

Target: SPARCL1 (SPARC-Like 1 (Hevin) (SPARCL1))

Alternative Name: Sparcl1

Background:

Synonyms: Ecm2, RAGS1 antigen, Extracellular matrix protein 2, Hevin, High endothelial venule protein, MAST 9, mast9, Matrix glycoprotein Sc1, PIG33, proliferation inducing protein 33, SC1, SPARC like 1, SPARC-like protein 1, Sparcl1, SPRL1_HUMAN.

Background: SPARC (secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. SPARC-like protein 1 (SPARCL1), also known as high endothelial venule protein (Hevin) or MAST9, is a 664 amino acid member of the SPARC family of proteins. Highly expressed in lymph node, heart, lung, brain, skeletal muscle, ovary, colon and small intestine, SPARCL1 is a secreted protein that contains one EF-hand domain, one follistatin-like domain and one Kazal-like domain. SPARCL1 is implicated to play a role in neuronal remodeling and tumor suppression. The gene encoding SPARCL1 maps to chromosome 4q22.1.

Gene ID: 8404