MUC5B antibody (Internal Region)

Catalog No. ABIN768619

This Goat Polyclonal antibody specifically detects MUC5B in ELISA and IF. It exhibits reactivity toward Mouse.

Quick Overview for MUC5B antibody (Internal Region) (ABIN768619)

Target: MUC5B (Mucin 5 Subtype B (MUC5B))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This MUC5B antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-MUC5B Antibody

Binding Specificity: Internal Region

Purpose: Muc5b (mouse)

Sequence: QHTYTHIDEC N

Specificity: EB06890

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence QHTYTHIDECN, from the internal region of the protein sequence according to NP_002449.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Not yet tested - our routine western blotting protocol does not allow for the detection of proteins >250 kDa.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in vesicles of NIH3T3 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MUC5B

Target: MUC5B (Mucin 5 Subtype B (MUC5B))

Alternative Name: Muc5b

Background: Muc5b, mucin 5, subtype B, tracheobronchial, 2300002I04Rik, A130042M24, AV085033, MUC5, MUC9, mucin 5b

Molecular Weight: 515kDa

Gene ID: 727897, 74180, 309114

NCBI Accession: NP_002449