IDH3B antibody (AA 369-383)

Catalog No. ABIN768624

This anti-IDH3B antibody is a Goat Polyclonal antibody detecting IDH3B in WB and ELISA. Suitable for Mouse.

Quick Overview for IDH3B antibody (AA 369-383) (ABIN768624)

Target: IDH3B (Isocitrate Dehydrogenase 3 (NAD+) beta (IDH3B))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IDH3B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-IDH3B Antibody

Binding Specificity: AA 369-383

Purpose: IDH3B (aa369-383)

Sequence: TTDFIKSVIG HLQTK

Specificity: This antibody is expected to recognize isoform a (NP_008830.2) and isoform c (NP_777281.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TTDFIKSVIGHLQTK, from the C Terminus of the protein sequence according to NP_008830.2, NP_777281.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 40 kDa band observed in lysates of Mouse fetal Kidney and fetal Skeletal Muscle and of cell line NIH3T3 (calculated MW of 42.2 kDa according to Human NP_008830.2 and Mouse NP_570954.1). Recommended concentration: 0.03-0.1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IDH3B

Target: IDH3B (Isocitrate Dehydrogenase 3 (NAD+) beta (IDH3B))

Alternative Name: IDH3B

Background: IDH3B, isocitrate dehydrogenase 3 (NAD+) beta, FLJ11043, H-IDHB, MGC903, RP46, NAD(+)-specific ICDH subunit beta, NAD+-specific ICDH, NAD+-specific isocitrate dehydrogenase b subunit, NAD+-specific isocitrate dehydrogenase beta, isocitrate dehydrogenase [

Molecular Weight: 42.2kDa according to Human NP_008830.2 and Mouse NP_570954.1

Gene ID: 3420, 170718, 94173

NCBI Accession: NP_008830, NP_777281