Smgc antibody (Internal Region)

Catalog No. ABIN768632

The Goat Polyclonal anti-Smgc antibody has been validated for ELISA and ICC. It is suitable to detect Smgc in samples from Mouse.

Quick Overview for Smgc antibody (Internal Region) (ABIN768632)

Target: Smgc (SMGC) (Submandibular Gland Protein C (SMGC))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Application: ELISA, Immunocytochemistry (ICC)

Grade: Verified

Product Details anti-Smgc Antibody

Binding Specificity: Internal Region

Purpose: Muc19 / Smgc

Sequence: KLEPKYENPT NGS

Cross-Reactivity: Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KLEPKYENPTNGS, from the internal region of the protein sequence according to NP_945121.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave bands at approx 65 kDa and 70 kDa in Mouse fetal Lung lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculat

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunocytochemistry: Positive staining of select epithelial cells in the murine Submanibular gland at E18. Recommended concentration 4ug/ml. Data provided by Everest Grant winner Melinda Larsen, State University of New York, Albany, NY.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Smgc

Target: Smgc (SMGC) (Submandibular Gland Protein C (SMGC))

Alternative Name: Smgc

Background: Smgc, submandibular gland protein C, 2310010P21Rik, DXImx49e, Muc19, Sfc21, OTTMUSP00000022832, neonatal submandibular gland protein C

Molecular Weight: 65kDa and 70kDa

Gene ID: 223809

NCBI Accession: NP_945121