ACPP antibody (AA 269-282)

Catalog No. ABIN768656

The Goat Polyclonal anti-ACPP antibody has been validated for WB, IHC and ELISA. It is suitable to detect ACPP in samples from Human.

Quick Overview for ACPP antibody (AA 269-282) (ABIN768656)

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ACPP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-ACPP Antibody

Binding Specificity: AA 269-282

Purpose: ACPP / PAP (aa269-282)

Sequence: NHMKRATQIP SYKK

Specificity: This antibody is expected to recognize both reported isoforms (NP_001127666.1, NP_001090.2).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NHMKRATQIPSYKK, from the internal region of the protein sequence according to NP_001127666.1, NP_001090.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Prostate shows reticulate cytoplasm staining, more intens towards the lumen of the gland. Recommended concentration, 3-5 μg/mL.

Western Blot: Approx 50 kDa band observed in Human Prostate lysates (calculated MW of 48.3 kDa according to NP_001127666.1). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ACPP

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Alternative Name: ACPP

Background: ACPP, acid phosphatase, prostate, ACP-3, ACP3, PAP, OTTHUMP00000216107, prostatic acid phosphatase, prostatic acid phosphotase

Molecular Weight: 48.3kDa according to NP_001127666.1

Gene ID: 55

NCBI Accession: NP_001127666, NP_001090

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process