CD83 antibody

Catalog No. ABIN775709

The Mouse Monoclonal anti-CD83 antibody is suitable to detect CD83 in samples from Human and Non-Human Primate. It has been validated for FACS and IHC (fro).

Quick Overview for CD83 antibody (ABIN775709)

Target: CD83

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD83 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: HB15e

Product Details anti-CD83 Antibody

Purpose: Anti-Hu CD83 Purified

Cross-Reactivity (Details): Human, Non-Human Primates

Characteristics: The mouse monoclonal antibody HB15e recognizes an extracellular epitope of CD83, a 40-45 kDa type I glycoprotein expressed on mature dendritic cells.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human CD83-transfected Cos cells

Isotype: IgG1, kappa

Application Details

Application Notes: Flow cytometry:Recommended dilution: 1-4 μg/mL, Positive control: peripheral blood-derived dendritic cells.
Immunohistochemistry (frozen sections): acetone fixation.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for CD83

Target: CD83

Alternative Name: CD83

Background: CD83 Molecule,CD83 is a 40-45 kDa heavily glycosylated type I cell surface glycoprotein of immunoglobulin family. It is expressed on the surface of mature dendritic cells, Langerhans cells in the skin, and interdigitating reticulum cells in the lymphoid tissues. Low expression of CD83 has been reported in activated T and B cells. Cytoplasmic expression of CD83 can be detected also in monocytes and macrophages. CD83 is involved in modulation of antigen presentation. Soluble CD83 has immunoregulatory functions, it is able to down-regulate dendritic cell maturation and stimulation of T cells. In the developing immune system, release of soluble CD83 from dendritic cells upon stimulation by gram-positive or gram-negative bacteria has anti-allergic effect. Herpes simplex virus, on the other hand, causes CD83 degradation in mature dendritic cells.,BL11, HB15

Gene ID: 9308

UniProt: Q01151