CD28 antibody

Catalog No. ABIN781619

The Mouse Monoclonal anti-CD28 antibody has been validated for FACS, WB, IF, IP, Func and IHC (fro). It is suitable to detect CD28 in samples from Human and Primate.

Quick Overview for CD28 antibody (ABIN781619)

Target: CD28

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: CD28-2

Product Details anti-CD28 Antibody

Specificity: This antibody reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.

Cross-Reactivity (Details): Species reactivity (tested):Human, Non-Human Primates

Purification: Protein A affinity chromatography

Purity: > 95 % (by SDS-PAGE)

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.Synonyms: T-cell-specific surface glycoprotein CD28, TP44

Gene ID: 940

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response