CD3 antibody

Catalog No. ABIN781714

This Mouse Monoclonal antibody specifically detects CD3 in FACS and IHC (fro). It exhibits reactivity toward Human and Rhesus Monkey.

Quick Overview for CD3 antibody (ABIN781714)

Target: CD3

Reactivity: Human, Rhesus Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: CRIS-7

Product Details anti-CD3 Antibody

Specificity: This antibody detects Human CD3. Antigen Distribution on Isolated cells: Peripheral T cells.

Cross-Reactivity (Details): Species reactivity (tested):Human, Rhesus Monkey.

Purification: Affinity Chromatography

Immunogen: Stimulated Human leukocytes

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL distilled water

Buffer: Stock solution contains 0.2 mg/mL IgG, PBS, pH 7.2 with 5 mg/mL BSA as stabilizer and 0.09 % Sodium Azide as a preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: The CD3 antigen is a protein complex composed of four distinct chains (CD3γ, CD3δ and two CD3ε) that associate with the T cell receptor (TCR) and the ζ-chain to generate an activation signal in T lymphocytes. The TCR, ζ-chain and CD3 Molecules together comprise the TCR complex. The CD3γ, CD3δ and CD3ε chains are highly related cell surface proteins of the immunoglobulin superfamily containing a single extracellular immunoglobulin domain.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gene ID: 916

NCBI Accession: NP_000724

UniProt: P07766

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway