Lactoferrin antibody (Biotin)

Catalog No. ABIN784363

This Goat Polyclonal antibody specifically detects Lactoferrin in WB, IP, IF, EIA, IHC (fro), ID and DB. It exhibits reactivity toward Monkey.

Quick Overview for Lactoferrin antibody (Biotin) (ABIN784363)

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Reactivity: Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This Lactoferrin antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunodiffusion (ID), Dot Blot (DB)

Product Details anti-Lactoferrin Antibody (Biotin)

Specificity: In immunoelectrophoresis against rhesus monkey milk a single precipitin line is obtained. The antiserum does not react with any other protein component of monkey serum or plasma. Cross-reactivity: The antiserum does not cross-react with any other monkey plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma or milk proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, however a strong cross-reaction with lactoferrin in human milk has been observed.

Characteristics: Molar Ratio: 6,2

Purification: DEAE-column Chromatography

Immunogen: Exocrine organs produce various secretions, each with its characteristic function. Proteins found in secretions may be divided into two groups: those specific for the particular secretion, and plasma proteins independent of the type of exocrine cells. Lactoferrin belongs to the first group. It is an iron containing protein with a molecular weight of 75,000 and it is antigenically different from transferrin. Lactoferrin has a slight anti-microbial action. Originally identified in milk, its presence has also been demonstrated in other secretions as saliva, semen and tears. The immunogen has been isolated from rhesus monkey milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 1 mL sterile distilled water

Concentration: 10 mg/mL

Buffer: PBS, pH 7.2 No preservative added, as it may interfere with the antibody activity. No foreign proteins added.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Lactoferrin

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Alternative Name: Lactotransferrin

Background: Lactoferrin is an iron binding glycoprotein with an approximate molecular weight of 80 kDa. The protein has two iron binding domains each housing one Fe3+ and the synergistic CO32- ion. The crystal structure form of human lactoferrin at 2.2A resolution exhibits 5330 protein atoms, 2Fe2+, 2CO32- and 98 carbohydrate atoms. Lactoferrin is absorbed from intestine by apical side of the membrane and localized to the nuclei. Intravenous infusion of lactoferrin is protective against lethal doses of E coli and induce bacterimia by a mechanism that downregulates neutrophil TNF alfa secretion. Recombinant human lactoferrin (rhLF), expressed and extracted from rice seed, is being evaluated for use as a dietary supplement to treat iron deficiency and/or iron deficiency induced anemia. Lactoferrin has been shown to have a role in the immune system and in early development of the embryo. A specific receptor for lactoferrin binding has been implicated in the human fetal intestine. Early embryonic localisation of lactoferrin by IHC has suggested its presence in various tissues including intestinal epitheliuem, kiney, and various regions of the brain.Synonyms: EC=3.4.21, LF, LTF, Lactoferrin, Talalactoferrin

Gene ID: 4057

NCBI Accession: NP_001186078

Pathways: Transition Metal Ion Homeostasis