GRIN2C antibody (pSer1096)

Catalog No. ABIN784607

The Rabbit Polyclonal anti-GRIN2C antibody has been validated for WB. It is suitable to detect GRIN2C in samples from Human, Mouse, Rat, Cow, Dog and Non-Human Primate.

Quick Overview for GRIN2C antibody (pSer1096) (ABIN784607)

Target: GRIN2C (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2c (GRIN2C))

Reactivity: Human, Mouse, Rat, Cow, Dog, Non-Human Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIN2C antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GRIN2C Antibody

Binding Specificity: pSer1096

Specificity: Specific for the ~140k NR2C subunit of the NMDA receptor phosphorylated at Ser1096. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide that was used to generate the antibody but not by the corresponding dephospho-peptide.

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Canine, Human and non-Human primates.
Species reactivity (tested):Mouse and Rat.

Purification: Affinity Chromatography

Immunogen: Synthetic phospho-peptide surrounding the phospho Ser1096 of the NR2C-subunit of Rat NMDA receptor.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C.

Target Details for GRIN2C

Target: GRIN2C (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2c (GRIN2C))

Alternative Name: NMDA Receptor 2C

Background: The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The NR2C subunit of the receptor is thought to influence the NMDAR conductance level (Ebralidze et al., 1996). Phosphorylation of Ser1096 by PKB on NR2C has been recently demonstrated to regulate NMDA receptor binding to 14-3-3 (Chen & Roche 2009)Synonyms: GRIN2C, Glutamate [NMDA] receptor subunit epsilon-3, N-methyl D-aspartate receptor subtype 2C, NMDAR2C, NR2C

Gene ID: 24411

NCBI Accession: NP_036707

Pathways: Synaptic Membrane