GAP43 antibody (AA 216-226)

Catalog No. ABIN789021

This anti-GAP43 antibody is a Rabbit Polyclonal antibody detecting GAP43 in WB, IHC and FACS. Suitable for Human, Rat, Mouse and Chicken.

Quick Overview for GAP43 antibody (AA 216-226) (ABIN789021)

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Reactivity: Human, Rat, Mouse, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAP43 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-GAP43 Antibody

Binding Specificity: AA 216-226

Cross-Reactivity: Chicken, Human, Mouse (Murine), Rat (Rattus)

Purification: affinity purified

Immunogen: Synthetic peptide corresponding to AA 216-226 of rat/mouse GAP-43, (C)-KEDPEADQEHA coupled to maleimide-activated KLH.

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: -20 °C

Target Details for GAP43

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Alternative Name: GAP43

Background: GAP-43 (growth associated protein 43 kDa, also known as neuromodulin) is a 24 kDa, neuron growth cone-associated member of the neuromodulin family. Apparently due to its tertiary structure, it runs anomalously at 43 kDa on SDS-polyacrylamide gel. Rat GAP-43 is a 226 amino acid (aa) protein that contains an N-terminal lipid binding motif, an 11 aa IQ domain (IQ:Ile-Gln-X-Ser-etc) (aa 38 - 48), and a C-terminus with four potential serine phosphorylation sites. The molecule may utilize the IQ domain to bind calmodulin. Protein kinase C-mediated phosphorylation of the Serine at 41 within this site inhibits this interaction. Unphosphorylated, calmodulin-bound GAP-43 is always associated with neurite retraction, while S41 phosphorylated GAP-43 stabilizes actin filaments and promotes growth cone extension.