Solute Carrier Family 14 (Urea Transporter, Kidney) Member 2 (SLC14A2) (AA 911-929) antibody

Catalog No. ABIN863205

Product Details

Target: Solute Carrier Family 14 (Urea Transporter, Kidney) Member 2 (SLC14A2)

Binding Specificity: AA 911-929

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)

Specificity: Isoform 1 and isoform 2 are expressed in the inner medulla of the kidney. Isoform 3 and isoform 4 are expressed in both the inner and outer renal medulla.,Detects ~97 and 127 kDa.

Cross-Reactivity: Mouse, Rat

Purification: Peptide Affinity Purified

Immunogen: Produced against a synthetic peptide mapped to the C-terminal tail (amino acids 911-929) of rat UT-A1 (antibody designation L194)

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:25)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN863205 was sufficient for detection of UT-A1 in 20 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: Solute Carrier Family 14 (Urea Transporter, Kidney) Member 2 (SLC14A2)

Alternative Name: UT-A1 (SLC14A2 Products)

Background: UT-A1, a kidney-specific urea transporter is expressed in the renal collecting duct where it mediates trans-epithelial urea transport and is a target for regulation by vasopressin. Urea movement out of the collecting duct in the inner medulla of the kidney allows accumulation of urea in the medullary interstitium, thereby allowing maximum water reabsorption from the collecting ducts. (The antibody also recognizes a second protein from the UT-A gene driven by transcription from an alternative promoter and expressed in the thin descending limb of Henle, viz. UT-A2) (1, 2).

Gene ID: 54302

NCBI Accession: NP_062220

UniProt: Q62668

Pathways: Response to Water Deprivation