Amyloid antibody

Catalog No. ABIN863211

This anti- antibody is a Rabbit Polyclonal antibody detecting in ELISA, IP, IHC, WB, ICC, IF and DB. Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for Amyloid antibody (ABIN863211)

Target: Amyloid

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Dot Blot (DB)

Product Details

Specificity: Recognizes generic epitopes common to many amyloid fibrils and fibrillar oligomers, but not prefibrillar oligomers or natively folded proteins. Expected to detect in Mouse and Rat based on homology.

Cross-Reactivity: Human

Purification: Protein A Purified

Immunogen: Fibrils prepared from human amyloid beta 42 peptide

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN863211 was sufficient for detection of amyloid fibrils on PVDF membranes using transferred fibrils by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References

Tang, Dai, He, Doty, Shultz, Sampson, Dai: "MEK guards proteome stability and inhibits tumor-suppressive amyloidogenesis via HSF1." in: Cell, Vol. 160, Issue 4, pp. 729-44, (2015) (PubMed).

Epelbaum, Youssef, Lacor, Chaurand, Duplus, Brugg, Duyckaerts, Delatour: "Acute amnestic encephalopathy in amyloid-β oligomer-injected mice is due to their widespread diffusion in vivo." in: Neurobiology of aging, Vol. 36, Issue 6, pp. 2043-52, (2015) (PubMed).

Target Details

Target: Amyloid

Background: Amyloid monomeric proteins can sometimes oligomerize into destructive amyloid fibrils. Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation. Many degenerative diseases are known to be related to the accumulation of misfolded proteins as amyloid fibres (1, 2). These include the amyloid-β peptide plaques and tau neurofibrillary tangles in senile plaques of Alzheimer's symptomology, the deposition of α-synuclein in the Lewy bodies of Parkinson's disease, and accumulation of polyglutamine-containing aggregates in Huntington's disease (2, 3).