Myosin IB antibody (Internal Region)

Catalog No. ABIN870635

The Goat Polyclonal anti-Myosin IB antibody has been validated for WB, IHC and ELISA. It is suitable to detect Myosin IB in samples from Human and Mouse.

Quick Overview for Myosin IB antibody (Internal Region) (ABIN870635)

Target: Myosin IB (MYO1B)

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Myosin IB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Myosin IB Antibody

Binding Specificity: Internal Region

Purpose: MYO1B

Sequence: RTVEAKQEKV STT

Specificity: This antibody is expected to recognize both reported isoforms (NP_001123630.1, NP_036355.2). Reported variants represent identical protein: NP_001123630.1, NP_001155291.1.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RTVEAKQEKVSTT, from the internal region of the protein sequence according to NP_001123630.1, NP_036355.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in the neuronal bodies. Recommended concentration, 5-10 μg/mL.

Western Blot: Approx 120 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 125 kDa according to Human NP_036355.2 and 128 kDa according to Mouse NP_034993.2). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Myosin IB

Target: Myosin IB (MYO1B)

Alternative Name: MYO1B

Background: MYO1B, myosin IB, myr1, MMI-alpha, MMIa, MYH-1c, MYO1B variant protein, OTTHUMP00000165861, myosin I alpha, myosin-I alpha, myosin-Ib

Molecular Weight: 125kDa

Gene ID: 4430, 17912, 117057

NCBI Accession: NP_001123630, NP_036355