TIRAP antibody (pTyr86)

Catalog No. ABIN872309

Product Details anti-TIRAP Antibody

Target: TIRAP (Toll-Interleukin 1 Receptor (TIR) Domain Containing Adaptor Protein (TIRAP))

Binding Specificity: pTyr86

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIRAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Dog,Cow,Pig,Horse,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic acetylpeptide derived from human TIRAP around phosphorylation site of Tyr86 [KD(p-Y)DV]

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-TIRAP antibody (ABIN872309)

Rolf, Kariminia, Ivison, Reid, Schultz: "Heterodimer-specific TLR2 stimulation results in divergent functional outcomes in B-cell precursor acute lymphoblastic leukemia." in: European journal of immunology, Vol. 45, Issue 7, pp. 1980-90, (2015) (PubMed).

Target Details for TIRAP

Target: TIRAP (Toll-Interleukin 1 Receptor (TIR) Domain Containing Adaptor Protein (TIRAP))

Alternative Name: TIRAP (TIRAP Products)

Background:

Synonyms: Mal, wyatt, BACTS1, MyD88-2, Toll/interleukin-1 receptor domain-containing adapter protein, TIR domain-containing adapter protein, Adaptor protein Wyatt, MyD88 adapter-like protein, TIRAP

Background: Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6.

Gene ID: 114609

UniProt: P58753

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades