Human Papilloma Virus 33 E7 (HPV-33 E7) (AA 41-97) antibody

Catalog No. ABIN872449

This Rabbit Polyclonal antibody specifically detects in IF (cc), IF (p), ELISA, IHC (fro) and IHC (p). It exhibits reactivity toward Human, Human Papillomavirus (HPV) and Virus.

Quick Overview for Human Papilloma Virus 33 E7 (HPV-33 E7) (AA 41-97) antibody (ABIN872449)

Target: Human Papilloma Virus 33 E7 (HPV-33 E7)

Reactivity: Human, Human Papillomavirus (HPV), Virus

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 41-97

Specificity: This antibody will also detect E7 in HPV58, but with slightly less affinity, due to a one amino acid change in the immunogen sequence range.

Cross-Reactivity: Human, Virus

Cross-Reactivity (Details): HPV33

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from HPV33 E7

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: Human Papilloma Virus 33 E7 (HPV-33 E7)

Alternative Name: HPV33 E7

Target Type: Viral Protein

Background:

Synonyms: E7 protein [Human papillomavirus type 33], Human Papilloma Virus, Human papillomavirus type 33, Human papillomavirus type 33, Protein 33, early protein E7 [Human papillomavirus type 33].

Background: E7 protein has both transforming and trans-activating activities. Disrupts the function of host retinoblastoma protein RB1/pRb, which is a key regulator of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interferes with histone deacetylation mediated by HDAC1 and HDAC2, leading to activation of transcription (By similarity).