PHLDA2 antibody (AA 31-80)

Catalog No. ABIN872459

The Rabbit Polyclonal anti-PHLDA2 antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect PHLDA2 in samples from Human and Rat. There are 2+ publications available.

Quick Overview for PHLDA2 antibody (AA 31-80) (ABIN872459)

Target: PHLDA2 (Pleckstrin Homology-Like Domain, Family A, Member 2 (PHLDA2))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHLDA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PHLDA2 Antibody

Binding Specificity: AA 31-80

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human TSSC3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-PHLDA2 antibody (ABIN872459)

Jin, Qiao, Luan, Li: "Lentivirus-mediated PHLDA2 overexpression inhibits trophoblast proliferation, migration and invasion, and induces apoptosis." in: International journal of molecular medicine, Vol. 37, Issue 4, pp. 949-57, (2016) (PubMed).

Jin, Qiao, Luan, Shang et al.: "The expression of the imprinted gene pleckstrin homology-like domain family A member 2 in placental tissues of preeclampsia and its effects on the proliferation, migration and invasion of trophoblast ..." in: Clinical and experimental pharmacology & physiology, Vol. 42, Issue 11, pp. 1142-51, (2015) (PubMed).

Target Details for PHLDA2

Target: PHLDA2 (Pleckstrin Homology-Like Domain, Family A, Member 2 (PHLDA2))

Alternative Name: TSSC3

Background:

Synonyms: IPL, BRW1C, BWR1C, HLDA2, TSSC3, Pleckstrin homology-like domain family A member 2, Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene C protein, Imprinted in placenta and liver protein, Tumor-suppressing STF cDNA 3 protein, Tumor-suppressing subchromosomal transferable fragment candidate gene 3 protein, p17-Beckwith-Wiedemann region 1 C, p17-BWR1C, PHLDA2

Background: Plays a role in regulating placenta growth. May act via its PH domain that competes with other PH domain-containing proteins, thereby preventing their binding to membrane lipids (By similarity).

Gene ID: 7262

UniProt: Q53GA4

Pathways: Regulation of Carbohydrate Metabolic Process