Perilipin A/B antibody

Catalog No. ABIN872948

The Rabbit Polyclonal anti- antibody has been validated for WB, FACS, IF (cc), IF (p), ELISA, IHC (fro) and IHC (p). It is suitable to detect in samples from Human and Mouse. There is 1 publication available.

Quick Overview for Perilipin A/B antibody (ABIN872948)

Target: Perilipin A/B

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Dog,Sheep,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Perilipin A+B

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References

James, Fox, Afsari, Lee, Clough, Knight, Ashmore, Ashton, Preham, Hoogduijn, Ponzoni, Hancock, Coles, Genever: "Multiparameter Analysis of Human Bone Marrow Stromal Cells Identifies Distinct Immunomodulatory and Differentiation-Competent Subtypes." in: Stem cell reports, Vol. 4, Issue 6, pp. 1004-15, (2015) (PubMed).

Target Details

Target: Perilipin A/B

Alternative Name: Perilipin A+B

Background:

Synonyms: Lipid droplet associated protein, PERIA, PERIB, Perilipin A, Perilipin, Perilipin B, PerilipinA, PerilipinB, PLIN.

Background: Perilipin is an intracellular neutral lipid droplet protein that is hormonally regulated. This protein is localized exclusively to the surface of lipid droplets. In response to lypotic stimuli, perilipin is phosphorylated by protein kinase A. Once activated, perilipin has inhibitory affects upon hormone-sensitive lipase (HSL), a protein that mediates the hydrolysis of triacylglycerol, the major form of stored energy in the body. Perilipin expression is limited to adipocytes and steroidogenic cells. There are currently two known isoforms, Perilipin A and B. Both of these proteins are encoded by a single-copy gene and are the result of differential splicing events.