SART1 antibody (AA 701-800)

Catalog No. ABIN873218

The Rabbit Polyclonal anti-SART1 antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect SART1 in samples from Human.

Quick Overview for SART1 antibody (AA 701-800) (ABIN873218)

Target: SART1 (Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SART1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SART1 Antibody

Binding Specificity: AA 701-800

Predicted Reactivity: Human,Mouse,Rat,Cow,Sheep,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SART1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for SART1

Target: SART1 (Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1))

Alternative Name: SART1

Background:

Synonyms: Ara 1, ARA1, HOMS 1, HOMS1, hSART 1, hSART1, hSnu 66, hSnu66, IgE autoantigen, MGC2038, SART 1, SART1 259, SART1259 protein, SART1800 protein, SART1259, Snu 66, Snu66, Squamous cell carcinoma antigen recognised by T cells, Squamous cell carcinoma antigen recognized by T cells 1, U4/U6.U5 tri snRNP associated 110 kDa protein, U4/U6.U5 tri snRNP associated protein, SNUT1_HUMAN.

Background: SART1 encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation, its full length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri snRNP to the pre spliceosome in the spliceosome assembly pathway.

Gene ID: 9092

Pathways: Ribonucleoprotein Complex Subunit Organization