alpha Fetoprotein antibody (AbBy Fluor® 350)

Catalog No. ABIN873515

The Mouse Monoclonal anti-alpha Fetoprotein antibody has been validated for WB and IF (p). It is suitable to detect alpha Fetoprotein in samples from Human and Rat.

Quick Overview for alpha Fetoprotein antibody (AbBy Fluor® 350) (ABIN873515)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Fetoprotein antibody is conjugated to AbBy Fluor® 350

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Clone: A2

Product Details anti-alpha Fetoprotein Antibody (AbBy Fluor® 350)

Cross-Reactivity: Human, Rat

Purification: Purified by Protein G.

Immunogen: Full length human AFP protein

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: AFP

Background:

Synonyms: AFPD, FETA, HPAFP, Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP

Background: Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Gene ID: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process