e4EBP1,2,3 (pThr45) antibody (AbBy Fluor® 350)

Catalog No. ABIN878105

The Rabbit Polyclonal anti- antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect in samples from Human.

Quick Overview for e4EBP1,2,3 (pThr45) antibody (AbBy Fluor® 350) (ABIN878105)

Target: e4EBP1,2,3

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: AbBy Fluor® 350

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details

Binding Specificity: pThr45

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human eIF4EBP1 around the phosphorylation site of Thr45

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: e4EBP1,2,3

Alternative Name: e4EBP1+2+3

Background:

Synonyms: p-4E-BP1/2/3 Thr 45, p-4EBP1/2/3Thr 45, e4EBP1 phospho T36, e4EBP1 phospho Thr36, p-e4EBP1 Thr36, e4EBP1, Eukaryotic translation initiation factor 4E binding protein 1, Eukaryotic translation initiation factor 4E-binding protein 1, 4E BP1, 4EBP1, BP 1, BP1, e4E binding protein 1, e4E-binding protein 1, Eukaryotic translation initiation factor 4E binding protein 1, MGC4316, PHAS I, PHASI, PHAS-I, PHAS, 4E-BP1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, Phosphorylated heat and acid stable protein regulated by insulin 1, 4EBP1_HUMAN.

Background: This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008].

Gene ID: 1978