Estrogen Receptor alpha antibody (AA 201-300) (Alexa Fluor 647)

Catalog No. ABIN895600

Product Details anti-Estrogen Receptor alpha Antibody

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Binding Specificity: AA 201-300

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Estrogen Receptor alpha antibody is conjugated to Alexa Fluor 647

Application: Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Predicted Reactivity: Sheep,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Estrogen Receptor alpha

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store at -20°C, for long storage, store at -80°C. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: Estrogen Receptor alpha (ESR1 Products)

Synonyms: LOC398734 antibody, AA420328 antibody, AU041214 antibody, ER-alpha antibody, ER[a] antibody, ERa antibody, ERalpha antibody, ESR antibody, Estr antibody, Estra antibody, Nr3a1 antibody, NR3A1 antibody, eralpha antibody, zfER[a] antibody, ER antibody, ESRA antibody, ESTRR antibody, Era antibody, Esr antibody, RNESTROR antibody, ERALPHA antibody, er antibody, esr antibody, esr1 antibody, nr3a1 antibody, XER antibody, era antibody, esra antibody, ERbeta antibody, xesr-1 antibody, xlERalpha1 antibody, xlERalpha2 antibody, ESR1 antibody, estrogen receptor 1 L homeolog antibody, estrogen receptor 1 (alpha) antibody, estrogen receptor 1 antibody, esr1.L antibody, Esr1 antibody, ESR1 antibody, esr1 antibody

Background:

Estrogen and progesterone receptor are members of a family of transcription factors that are regulated by the binding of their cognate ligands. The interaction of hormone-bound estrogen receptors with estrogen responsive elements(EREs) alters transcription of ERE-containing genes. The carboxy terminal region of the estrgen receptor contains the ligand binding domain, the amino terminus serves as the transactivation domain, and the DNA binding domain is centrally located. Two forms of estrogen receptor have been identified, ER Alpha and ER Beta. ER Alpha and ER Beta have been shown to be differentially activated by various ligands. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (hPR-A and hPR-B), which arise from alternative splicing. In most cells, hPR-B functions as a transcriptional activator of progesterone-responsive gene, whereas hPR-A function as a transcriptional inhibitor of all steroid hormone receptors.

Subcellular location: Cytoplasm, Nucleus, Cell membrane

Synonyms: Estradiol receptor, Estrogen receptor alpha, Estradiol Receptor-alpha, Estrogen Receptor 1, Atherosclerosis, susceptibility to, included, DKFZp686N23123, ER Alpha, ER, ER-alpha, ERalpha, ER[a], Era, ESR, ESR1, ESR1_HUMAN, ESR2, ESRA, Estr, Estrogen receptor 1 alpha, Estrogen resistance, included, HDL cholesterol, augmented response of, to hormone replacement, included, Myocardial infarction, susceptibility to, included, NR3A1, Nuclear receptor subfamily 3 group A member 1, OTTHUMP00000017718, OTTHUMP00000017719, RNESTROR.

Gene ID: 2099

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly