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Estrogen Receptor alpha antibody (AA 233-483)

ESR1 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7433072
  • Target See all Estrogen Receptor alpha (ESR1) Antibodies
    Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))
    Binding Specificity
    • 32
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    AA 233-483
    Reactivity
    • 481
    • 173
    • 166
    • 19
    • 18
    • 10
    • 7
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Mouse
    Host
    • 391
    • 97
    • 2
    • 1
    Rabbit
    Clonality
    • 371
    • 122
    Polyclonal
    Conjugate
    • 222
    • 35
    • 24
    • 22
    • 19
    • 15
    • 15
    • 15
    • 15
    • 15
    • 15
    • 11
    • 8
    • 8
    • 8
    • 8
    • 8
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Estrogen Receptor alpha antibody is un-conjugated
    Application
    • 376
    • 172
    • 142
    • 119
    • 97
    • 94
    • 80
    • 67
    • 39
    • 37
    • 24
    • 15
    • 10
    • 7
    • 7
    • 6
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Purpose
    Polyclonal Antibody to Estrogen Receptor Alpha (ERa)
    Specificity
    The antibody is a rabbit polyclonal antibody raised against ERa. It has been selected for its ability to recognize ERa in immunohistochemical staining and western blotting.
    Cross-Reactivity
    Human, Rat
    Purification
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogen
    Recombinant Estrogen Receptor Alpha (ERa) corresdonding to Ile233~Leu483 with N-terminal His and GST Tag
    Isotype
    IgG
    Top Product
    Discover our top product ESR1 Primary Antibody
  • Application Notes

    Western blotting: 0.5-2 μg/mL

    1:500-2000 Immunohistochemistry: 5-20 μg/mL

    1:50-200 Immunocytochemistry: 5-20 μg/mL

    1:50-200 Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Expiry Date
    24 months
  • Target
    Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))
    Alternative Name
    Estrogen Receptor Alpha (ESR1 Products)
    Background
    ER-A1, ER, ESR, ESR1, ESRA, NR3A1, NR3-A1, Estrogen Receptor 1, Nuclear Receptor Subfamily 3,Group A,Member 1
    Pathways
    Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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