Myogenin antibody (AbBy Fluor® 350)

Catalog No. ABIN907471

Product Details anti-Myogenin Antibody (AbBy Fluor® 350)

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Reactivity: Human, Mouse, Rat, Pig, Cow, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myogenin antibody is conjugated to AbBy Fluor® 350

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Cow, Human, Mouse, Rat

Predicted Reactivity: Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MYOG/Myogenin

Isotype: IgG

Application Details

Application Notes: IF(IHC-P)(1:100-500)
Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Myogenin

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Alternative Name: Myogenin (MYOG Products)

Background:

Synonyms: MYF4, myf-4, bHLHc3, Myogenin, Class C basic helix-loop-helix protein 3, Myogenic factor 4, MYOG

Background: Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth, phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts (By similarity).

Molecular Weight: 25kDa

Gene ID: 4656

UniProt: P15173

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development