SIRT4 antibody (AA 51-150) (Biotin)

Catalog No. ABIN915295

This Rabbit Polyclonal antibody specifically detects SIRT4 in WB, ELISA and IHC (p). It exhibits reactivity toward Mouse and Rat.

Quick Overview for SIRT4 antibody (AA 51-150) (Biotin) (ABIN915295)

Target: SIRT4 (Sirtuin 4 (SIRT4))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT4 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SIRT4 Antibody (Biotin)

Binding Specificity: AA 51-150

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Cow,Sheep,Pig,Horse,Rabbit,Zebrafish,Drosophila

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SIRT4/SIR 2 like protein 4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for SIRT4

Target: SIRT4 (Sirtuin 4 (SIRT4))

Alternative Name: SIRT4

Background:

Synonyms: SIR2L4, NAD-dependent protein deacetylase sirtuin-4, NAD-dependent ADP-ribosyltransferase sirtuin-4, Regulatory protein SIR2 homolog 4, SIR2-like protein 4, SIRT4

Background: Acts both as NAD-dependent protein ADP-ribosyl transferase and NAD-dependent protein deacetylase. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest. In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor. Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis. Down-regulates insulin secretion.

Gene ID: 23409

UniProt: Q9Y6E7

Pathways: Negative Regulation of Hormone Secretion