SPARC antibody (PE-Cy5.5)

Catalog No. ABIN916170

This anti-SPARC antibody is a Rabbit Polyclonal antibody detecting SPARC in WB. Suitable for Human, Mouse, Rat, Cow, Pig and Dog.

Quick Overview for SPARC antibody (PE-Cy5.5) (ABIN916170)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPARC antibody is conjugated to PE-Cy5.5

Application: Western Blotting (WB)

Product Details anti-SPARC Antibody (PE-Cy5.5)

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SPARC

Isotype: IgG

Application Details

Application Notes: WB(1:100-500)
FCM(1:100-500)
IHC-P(1:100-500)
IF(IHC-P)(1:100-500)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: SPARC

Background:

Synonyms: ON, SPARC, Basement-membrane protein 4, BM-4, Osteonectin, Secreted protein acidic and rich in cysteine

Background: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites, an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Molecular Weight: 33kDa

Gene ID: 6678

UniProt: P09486

Pathways: Autophagy