WT1 antibody (N-Term)

Catalog No. ABIN927690

This anti-WT1 antibody is a Rabbit Polyclonal antibody detecting WT1 in WB. Suitable for Human.

Quick Overview for WT1 antibody (N-Term) (ABIN927690)

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-WT1 Antibody

Binding Specificity: N-Term

Purification: Purified

Immunogen: WT1 antibody was raised in rabbit using the N terminal of WT1 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

WT1 Blocking Peptide, (ABIN5617029), is also available for use as a blocking control in assays to test for specificity of this WT1 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: WT1

Background: WT1 is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Synonyms: Polyclonal WT1 antibody, Anti-WT1 antibody, Wilms tumor 1 antibody, AWT1 antibody, GUD antibody, WAGR antibody, WIT-2 antibody, WT33 antibody.

Pathways: Tube Formation