XBP1 antibody

Catalog No. ABIN930955

This Mouse Monoclonal antibody specifically detects XBP1 in DB. It exhibits reactivity toward Human.

Quick Overview for XBP1 antibody (ABIN930955)

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This XBP1 antibody is un-conjugated

Application: Dot Blot (DB)

Clone: XBP1H6E5

Product Details anti-XBP1 Antibody

Cross-Reactivity: Human

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: XBP1 antibody was raised in mouse using recombinant X-Box Binding Protein 1

Isotype: IgG1

Application Details

Application Notes: Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: XBP1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for XBP1

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Alternative Name: XBP1

Background: This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. Synonyms: Monoclonal XBP1 antibody, Anti-XBP1 antibody, Xbox binding protein 1 antibody, XBP2 antibody, TREB5 antibody.

Pathways: ER-Nucleus Signaling, Unfolded Protein Response