DHX29 antibody

Catalog No. ABIN931040

The Mouse Monoclonal anti-DHX29 antibody has been validated for WB, IP, FACS, ICC and DB. It is suitable to detect DHX29 in samples from Human.

Quick Overview for DHX29 antibody (ABIN931040)

Target: DHX29 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 29 (DHX29))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DHX29 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Dot Blot (DB)

Clone: 2269C1

Product Details anti-DHX29 Antibody

Cross-Reactivity: Human

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: DHX29 antibody was raised in mouse using recombinant Human Deah (Asp-Glu-Ala-His) Box Polypeptide 29 (Dhx29)

Isotype: IgG1

Application Details

Application Notes: WB: 0.2-2 µg/mL, IP: 100-500 µg/sample, FC: 0.5-2 µg/sample, ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: DHX29 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for DHX29

Target: DHX29 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 29 (DHX29))

Alternative Name: DHX29

Background: DHX29 encodes an ATP-binding RNA helicase involved in translation initiation. Required for efficient initiation on mammalian mRNAs with structured 5'-UTRs by promoting efficient NTPase-dependent 48S complex formation. Specifically binds to the 40S ribosome near the mRNA entrance. Does not possess a processive helicase activity. Synonyms: Monoclonal DHX29 antibody, Anti-DHX29 antibody, DEAH (Asp-Glu-Ala-His) box polypeptide 29 antibody, DDX29 antibody, FLJ21492 antibody.