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- Target
- NPT
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
- Application
- ELISA, Western Blotting (WB)
- Immunogen
- NPT antibody was raised in Mouse using a purified recombinant fragment of NPT expressed in E. coli as the immunogen.
- Clone
- 4B4D1
- Isotype
- IgG2a
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anti-NPT antibody
Reactivity: Human ELISA Host: Mouse Monoclonal 4B4D1 unconjugated
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- Application Notes
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ELISA: 1:10,000, WB: 1:500-1:2000
Optimal conditions should be determined by the investigator. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied as a liquid in ascitic fluid containing 0.03 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Handling Advice
-
Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4 °C for short term storage. Store at -20 °C for long term storage.
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- Target
- NPT
- Background
- NPT is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPT is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents. Synonyms: Monoclonal NPT antibody, Anti-NPT antibody, Neomycin phosphotransferase II antibody, NptII antibody.
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