NPT (truncated) antibody

Catalog No. ABIN2464088

Product Details

Target: NPT

Binding Specificity: truncated

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Purification: Antibody is purified by protein A affinity chromatography.

Immunogen: Ni-NTA purified truncated recombinant NPT expressed in E. Coli strain BL21 (DE3).

Clone: 4B4D1

Isotype: IgG2a

Application Details

Application Notes: Western Blot:Dilution 1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: -20 °C

Storage Comment: NPT monoclonal antibody can be stored at -20 °C, stable for one year.

Target Details

Target: NPT

Background: Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.

Gene ID: 11979140

UniProt: Q79C30